Aktogen
Associative learning and memory Chemosensation Courtship behaviour Motility
Non-associative learning and memory Olfaction Phototaxis

It is not an unusual situation that a scientist with a few years of effort generates and tests mutant Drosophila lines just to discover that they do not show any detectable phenotype in the planned assays. These fly lines might look a bit sluggish, might not readily fly, or have other, at first sight undetectable behaviour abnormalities (like learning or memory defects) but these features are hard to quantify and frequently perceived as outside of the scope of the investigation. Yet, these “irrelevant” phenotypes can lead to truly novel discoveries or at least their characterisation can make years of apparently fruitless efforts publishable.

Many fruit fly laboratories lack the scientific knowledge and equipment for doing proper behaviour tests. It can be the most time and cost effective solution to find the right expertise elsewhere. Aktogen Limited offers behaviour test and related services for Drosophila laboratories that need fast and reliable answers to their questions in this field. Based on Dr. Zoltan Asztalos’ extensive experience in fruit fly behaviour genetics (see short CV in the Appendix) and the mostly automated behaviour systems the company developed or has access to, Aktogen can guarantee to generate behaviour data at the highest scientific standard.

Aktogen offers a range of Drosophila behaviour tests from as “simple” as locomotion to as complex as associative learning and memory. One can choose the most appropriate single test for the given mutant or subject it to a battery of tests when there is no obvious clue to choose one test or a thorough behaviour examination is needed.

A typical behaviour experiment comprises the following steps:
Bring fly lines through a quarantine procedure (minimum 6 weeks)
Breed larvae/adults of the fly lines in temperature and humidity controlled rooms
- synchronize egg laying to have same developmental phase of the larvae to be compared
- use same number of parents for set time egg laying to control crowding (larval/adult size affects behaviour)

Collect, sex, clean, count and age larvae/adults appropriately

Create and test experimental set-ups with own wild-type flies to see if paradigms work (in the behaviour room light, temperature and humidity are controlled)
Experimental repeats are done on different days to avoid effect of "daily variation"
Collect behaviour data and do statistical analysis (determine if sample size was large enough to have statistically significant difference between relevant lines, if not, repeat experiments)

Write report

Aktogen guarantees to
make all used experimental protocols available for evaluation and publication
do follow-up experiments for the equivalent price
be available for writing or communicating papers (prepared to be author on papers)
Please fill in our 'First contact questionnaire ' to facilitate understanding of your experimental requirements and preparing a quotation."

Download: First contact questionnaire

Associative learning and memory Chemosensation Courtship behaviour Motility
Non-associative learning and memory Olfaction Phototaxis

It is not an unusual situation that a scientist with a few years of effort generates and tests mutant Drosophila lines just to discover that they do not show any detectable phenotype in the planned assays. These fly lines might look a bit sluggish, might not readily fly, or have other, at first sight undetectable behaviour abnormalities (like learning or memory defects) but these features are hard to quantify and frequently perceived as outside of the scope of the investigation. Yet, these “irrelevant” phenotypes can lead to truly novel discoveries or at least their characterisation can make years of apparently fruitless efforts publishable.

Many fruit fly laboratories lack the scientific knowledge and equipment for doing proper behaviour tests. It can be the most time and cost effective solution to find the right expertise elsewhere. Aktogen Limited offers behaviour test and related services for Drosophila laboratories that need fast and reliable answers to their questions in this field. Based on Dr. Zoltan Asztalos’ extensive experience in fruit fly behaviour genetics (see short CV in the Appendix) and the mostly automated behaviour systems the company developed or has access to, Aktogen can guarantee to generate behaviour data at the highest scientific standard.

Aktogen offers a range of Drosophila behaviour tests from as “simple” as locomotion to as complex as associative learning and memory. One can choose the most appropriate single test for the given mutant or subject it to a battery of tests when there is no obvious clue to choose one test or a thorough behaviour examination is needed.

A typical behaviour experiment comprises the following steps:
Bring fly lines through a quarantine procedure (minimum 6 weeks)
Breed larvae/adults of the fly lines in temperature and humidity controlled rooms
- synchronize egg laying to have same developmental phase of the larvae to be compared
- use same number of parents for set time egg laying to control crowding (larval/adult size affects behaviour)

Collect, sex, clean, count and age larvae/adults appropriately

Create and test experimental set-ups with own wild-type flies to see if paradigms work (in the behaviour room light, temperature and humidity are controlled)
Experimental repeats are done on different days to avoid effect of "daily variation"
Collect behaviour data and do statistical analysis (determine if sample size was large enough to have statistically significant difference between relevant lines, if not, repeat experiments)

Write report

Aktogen guarantees to
make all used experimental protocols available for evaluation and publication
do follow-up experiments for the equivalent price
be available for writing or communicating papers (prepared to be author on papers)
Please fill in our 'First contact questionnaire ' to facilitate understanding of your experimental requirements and preparing a quotation."

Download: First contact questionnaire